AffiCLEAN® MycoX Removal Mix
AffiCLEAN® MycoX™ Removal Mix is a highly effective mycoplasma removal solution designed to eliminate mycoplasma contamination from cell cultures. Utilizing a dual-action mechanism, it efficiently targets and removes both extracellular and intracellular mycoplasma, ensuring your cultures remain pure and uncontaminated. While Plasmocin is another popular choice for mycoplasma elimination, AffiCLEAN® MycoX™ offers several distinct advantages, making it the superior option for cell culture contamination control.
Key Features of AffiCLEAN® MycoX™ Removal Mix : - Dual-Action Formula : Combines two potent antibiotics to effectively remove mycoplasma contamination from both extracellular and intracellular sources.
- Preserves Cell Viability : Specifically designed to minimize cytotoxicity, ensuring that your cell cultures remain healthy and viable after mycoplasma removal.
- Proven Efficacy : Effective against a broad spectrum of mycoplasma species, providing comprehensive coverage for your cell cultures.
- Easy-to-Use : Simple application process with no complex steps, making it an ideal solution for routine use in labs and research settings.
Key Features of AffiCLEAN® MycoX™ Removal Mix : - Dual-Action Formula : Combines two potent antibiotics to effectively remove mycoplasma contamination from both extracellular and intracellular sources.
- Preserves Cell Viability : Specifically designed to minimize cytotoxicity, ensuring that your cell cultures remain healthy and viable after mycoplasma removal.
- Proven Efficacy : Effective against a broad spectrum of mycoplasma species, providing comprehensive coverage for your cell cultures.
- Easy-to-Use : Simple application process with no complex steps, making it an ideal solution for routine use in labs and research settings.
AffiCLEAN® MycoX™ Removal Mix | Powerful Mycoplasma Elimination Solution for Cell Cultures
AffiCLEAN® MycoX™ Removal Mix is a broad-spectrum antimicrobial reagent designed to eliminate mycoplasma contamination from mammalian cell cultures. In its 1000× concentrated format, it ensures economical, long-term use while maintaining high efficacy and low cytotoxicity. This versatile solution is ideal for both adherent and suspension cell lines, enabling complete decontamination typically within 2–3 weeks without affecting cell viability.
Key Features of AffiCLEAN® MycoX™ Removal Mix
- Broad-Spectrum Activity : Targets over 70 mycoplasma species, ensuring comprehensive mycoplasma removal from your cell cultures.
- Cell-Safe Formula : Non-cytotoxic to commonly used mammalian cells, allowing for effective decontamination without damaging your cultures.
- Fast Decontamination : Achieves complete mycoplasma clearance in 4–6 passages (approximately 2–3 weeks), saving time and preserving cell health.
- Ready-to-Use Format : Simply dilute 1 μL of AffiCLEAN® MycoX™ into 1 mL of culture medium for easy integration into your workflow.
- Cost-Efficient : 1 mL of AffiCLEAN® MycoX™ treats up to 1 liter of culture, making it an affordable solution for long-term use in large-scale applications.
Applications of AffiCLEAN® MycoX™ Removal Mix
- Decontamination of Mycoplasma-Infected Cell Lines : Effectively eliminates mycoplasma contamination, restoring the purity of your cultures.
- Rescue of Valuable Cell Stocks : Ideal for mycoplasma-contaminated cell lines, AffiCLEAN® MycoX™ allows you to recover valuable stocks without starting over.
- Routine Cleanup in Labs : Perfect for academic, biotech, and GMP research labs to maintain mycoplasma-free cell cultures.
- Preparation of Mycoplasma-Free Master Cell Banks : Essential for ensuring mycoplasma-free stock cultures, ready for future use in research or production.
Kit Components
- (1 Vial) AffiCLEAN® MycoX™ Removal Mix (Detailed kit components are provided in the datasheet)
Recommended Protocol
- Dilution : Add 1 μL of MycoX™ to 1 mL of culture medium (final 1× concentration).
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Treatment Schedule :
- Apply continuously for 4–6 cell passages (approx. 2–3 weeks).
- Replace media every 2–3 days with fresh AffiCLEAN® MycoX™.
- Post-Treatment : Validate decontamination using AffiPCR® or AffiZYME® Mycoplasma Detection Kits to confirm mycoplasma-free cultures.
Storage & Stability
- Storage : Store between 2–8°C (refrigerated).
- Shelf Life : 12 months unopened.
- Do not freeze.
Quality Control
- Each batch is rigorously tested for sterility and mycoplasma elimination efficiency.
- Validated against a panel of common mycoplasma strains to ensure consistency and performance.
Why Choose AffiCLEAN® MycoX™ Removal Mix ?
- Highly Effective Mycoplasma Elimination : AffiCLEAN® MycoX™ is proven to effectively clear mycoplasma contamination from cell cultures without harming the cells, preserving your research integrity.
- Cost-Efficient : With a 1000× concentrated format, AffiCLEAN® MycoX™ provides long-term and affordable mycoplasma removal for large-scale labs.
- Trusted Solution for Research and Production : Whether you're in biotech, pharmaceuticals, or academic research, AffiCLEAN® MycoX™ ensures the purity of your cell lines and master cell banks.
Plasmocin vs. AffiCLEAN® MycoX™ Removal Mix :
Key Differences
While both Plasmocin and AffiCLEAN® MycoX™ are used for mycoplasma elimination, there are distinct differences in their formulations and application.
1- Broad-Spectrum Action
- AffiCLEAN® MycoX™ utilizes a dual-action mechanism to target both extracellular and intracellular mycoplasma, making it more effective in thoroughly eliminating contamination from your cell cultures.
- Plasmocin, on the other hand, is primarily effective at eliminating extracellular mycoplasma and may not target intracellular contamination as efficiently.
2- Cell Viability
- AffiCLEAN® MycoX™ is specially formulated to ensure minimal cytotoxicity, preserving the viability of your cell cultures after treatment. This is crucial for maintaining cell integrity and supporting further experimental use.
- Plasmocin has a more potent cytotoxic effect, which can sometimes affect cell viability, especially with prolonged use or at higher concentrations.
3- Ease of Use
- AffiCLEAN® MycoX™ is designed to be easy to use with a simplified application process that reduces handling time and complexity.
- Plasmocin can sometimes require more careful application and may involve additional steps to ensure optimal mycoplasma removal.
4- Treatment Time:
- AffiCLEAN® MycoX™ typically shows results within a shorter time frame (around 2 weeks), providing faster elimination of mycoplasma contamination.
- Plasmocin may require longer treatment times to fully clear contamination, especially when dealing with intracellular mycoplasma.